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Radioligand binding studies were performed with 125I-BOP.
Competition binding studies were performed in close analogy to the manufacturer's protocol.
UV Vis spectral analysis and DNA binding studies were performed on a JASCO, V-650 spectrophotometer.
125I CCK-8 binding and [35S]guanosine triphosphate γS (GTPγS) binding studies were performed.
The binding studies were performed by UV vis and NMR methods.
In vivo competitive binding studies were performed to investigate the specificity of 111In-DTPA-11B6 to hK2.
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The second set of binding studies was performed using CD20/CD22 double positive Raji and Ramos cells.
The first set of binding studies was performed on 293T cells (CD20/CD22 negative) transfected with CD20 or CD22.
Mechanistic heme-binding studies were performed to identify the mechanism of action of the synthesized molecules and good binding interactions were observed.
Competitive real-time cell-binding studies were performed on CHO-K1 (negative control) and CHO-K1-hA3R cells in presence of 0.7 nM [125I]-AB-MECA at ambient temperature with LigandTracer® Grey (Ridgeview Instruments AB, Uppsala, Sweden).
Recombinant human Epo-binding studies were performed as previously described (Elliott et al, 1997) with some modifications.
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