Sentence examples for binding studies were expressed from inspiring English sources
CIDRα1 domains for binding studies were expressed in baculovirus-infected High Five cells and purified by metal affinity chromatography.
All PINK1 enzymes used in this study were expressed in Escherichia coli as full‐length maltose‐binding protein fusion proteins as previously described.
Competition binding studies were performed by incubating membranes expressing hCB1R or hCB2R with unlabeled compounds and tritiated CP 55,940 (0.5 nM) or WIN-55212 (0.8 nM) respectively, for two hours at 37 °C.
Binding values were expressed relative to membrane protein.
Cell-binding studies were conducted on adherent Chinese hamster ovary cells (CHO-K1) and CHO-K1 cells stably expressing the human adenosine A3 receptor (CHO-K1-hA3R).
Data were expressed relative to binding of the control cell line and are average of three separate experiments.
The antibody fragments were expressed, purified and their binding specificity determined towards rMERS-NP.
D174N, E178Q, and E256Q mutants were expressed and dialyzed to remove putrescine and studied biochemically using X-ray crystallography, UV-CD spectroscopy, analytical ultracentrifugation, and ITC binding studies.
All chimeric receptors were expressed at similar levels, had similar binding affinities for EPO, and conferred EPO-dependent cell growth.
The stepwise and overall binding constants for Ca2+ binding to NCaBD are expressed as follows.
