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Competition binding studies were done mainly as previously described [55].
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A competitive radioactive binding study was done to ascertain whether PME interacts with ER and had shown that PME binds to ER and inhibited the binding of labelled estrogen to ER in a dose-dependent manner [ 53, 91].
In initial efforts to measure levels of circulating FRα by 3H-FA binding studies, preliminary studies were done to determine the attributes of the microcentrifuge filtration assay.
The only TF tested in any detail is REST, with one Figure panel pertaining to Oct4 and no studies were done using protein binding or other functional assays, so it is based on presence of binding sites only.
Overall, there are only a few references on in vitro endotoxin binding studies which were done using the kinetic chromogenic LAL test, and most of these studies were done with other sorbents than clay minerals.
Isothermal Titration Calorimetric (ITC) binding studies were also done with redADan peptide and αA-crystallin.
Cell viability assay was done by MTT-assay while the binding studies were carried out using fluorescence spectroscopy, circular diachroism, molecular modelling and computational analysis.
Saturation binding studies were carried out to analyse the in vitro binding properties of the diabody.
All binding assays were done in duplicate.
All binding assays were done in triplicates.
If components of this system sense and bind CAMPs (page 3) to effect a change, perhaps some of these binding studies should have been done in the presence as well as absence of CAMPs.
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