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Autoradiograms from ISH and receptor binding studies were analyzed using the optical density readings (grey intensity).
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Survival studies were analyzed using log-rank test.
Overall, 58 studies were analyzed.
The diazepine derivatives derived in the present study were analyzed for the binding affinity with NS5B polymerase.
SPR binding studies were performed with a BIAcore 3000 instrument (GE Healthcare) and were analyzed using the software package Scrubber2 (Biologic Software).
UTP, GTP, and AMP binding studies were carried out for selected aptamers identically to the ATP studies to analyze the impact on specificity and selectivity.
Each study was analyzed separately.
VP-ITC calorimeter (MicroCal) was used for all ligand binding studies and data were analyzed by MicroCal LLC ITC software (MicroCal).
The relative levels of EZH2 binding activities were analyzed by real-time PCR.
Region-specific binding affinities were analyzed using Student's unpaired t-test.
(A) The levels of H3K4me3, H3K9me3 and H3K27me3 of the C/EBPβ binding region were analyzed by ChIP assay.
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