The levels of TSP binding to M3Da. cells evaluated by binding studies, using an anti-TSP monoclonal antibody (MAb) (LYP8), shows 367,000 +/- 58,000 (mean +/- s.d).
In a binding study using a DC-SIGN-expressing cell line, the lectin showed a high preference for the Mycobacterium spp. from the tuberculosis complex, while it bound little or not to strains from outside the complex (Pitarque et al., 2005).
As a proof of concept for imaging differentiated thyroid cancer in human, we performed ex-vivo binding studies using as target a fresh metastatic cervical lymph node excised from a patient bearing a papillary thyroid carcinoma.
On the basis of the ability of MIBE to interact with GPER in docking simulations, we also performed ligand binding studies using radiolabeled E2 as a tracer in ER-negative but GPER-positive SkBr3 breast cancer cells, as previously reported [ 28].
Binding studies using microcrystalline cellulose (Avicel) and a commercial source of cellulose acetate confirmed functional production of the CtCBM3 domain; affinity gel electrophoresis using acetylated xylan also verified the selectivity of CtCBM3 binding to cellulose.
In vitro binding studies using purified GST-Gravin fragments mapped a central region of the anchoring protein (amino acids 451 to 900) that directly interacts with Aurora A. More detailed mapping studies defined at least two extended Aurora A-binding sequences within this region, thus eliminating site-directed mutagenesis as the most direct approach to disrupt the Aurora A-Gravin interaction.
The relative affinity of ERRα for the wild-type and variant sequences was assessed in competition binding studies using increasing amounts of unlabeled G or A dsDNA probe to displace wild-type G probe in the EMSA binding reactions.
This was established in binding studies using the full size OpuA protein in a detergent-solubilized state as well as the isolated SBD domains expressed as soluble protein (OpuAC).
In binding studies using [3H]vinblastine, XR9051 was shown to be a potent inhibitor of the binding of the cytotoxic to P-glycoprotein (EC50 = 1.4 +/- 0.5 nM).
To understand better the regulation of SNF1 by ADP, we did a series of binding studies using the phosphorylated, active form of the enzyme.
