In in vitro binding studies on U87MG human glioma cells, the linker-containing dimers HYNIC-3PEG4-dimer (IC50 = 60 ± 4 nM) and HYNIC-3G3-dimer (IC50 = 61 ± 2 nM) had a higher affinity than HYNIC-PEG4-dimer (IC50 = 84 ± 7 nM), which had a short linker between the RGD units.
Binding studies on MCF-7 human breast cancer cells (2) were performed after each structural modification to make sure that the potency and the selectivity of the new NOTA-peptide conjugates to NPY-Y1R were maintained.
The fortuitous absence of tryptophan in GroEL, combined with the unusually high tryptophan content of TSPO (13 of 169 amino acids), made it possible to conduct some ligand binding studies with the human protein based on fluorescence quenching, but not to characterize its oligomeric state.
This is a comprehensive mass spectrometric report on binding study of TMPyP4 with human telomeric DNA/RNA G-quadruplexes.
This is a comprehensive and comparative mass spectrometric report on binding study of TMPyP4 with human telomeric DNA/RNA G-quadruplexes.
A comparative study on human telomeric DNA G-quadruplex binding of meso-5,10,15,20-tetrakis N-methyl-4-pyridyl porphyrin meso-5,10,15,20-tetrakis N-methyl-4-pyridyl porphyrinate and tetrachloride, was conducted by using ESI-TOF-meso-5,10,15,20-tetrakis N-methyl-4-pyridyl porphyrinthods.
Notably, we used the same optimized oligonucleotide probes to investigate DNA binding of HNF6 as reported in our initial study on human colorectal liver metastases [3].
The binding study results were confirmed on human CXCR4-expressing SupT1 cells and correlated with entry efficiency using a virus entry assay.
Studies on the mapping of the binding side on human immunoglobulins suggested that the Fc interactions are, in some respects, very similar across species [31].
Studies on the mapping of the binding side on human immunoglobulins suggested that the Fc interactions are, in some respects, very similar across species [ 31].
