However, our competition binding study did not show any binding of SA and 3,10DDA and binding only at extreme concentrations (10−4 M) of 10H2DA, indicating that an interaction with ERs is not mediated via the ligand binding pocket.
Supporting IP data also show that Tat expression can "rescue" the effects of aff4 mutations that would otherwise inhibit Cyclin T1 binding, although these studies do not unambiguously identify aff4 residues that contact Tat (Glu61/Met62 are presented as attractive candidates, but the binding/rescue effects are small).
Comparison of pyrethroid effects on QNB binding across studies does not reveal clear trends in reported effects between laboratories.
Quantitative EMSA analysis showed robust binding between EZH2 alone and the HOTAIR 5′ domain with Kd = 755 ± 43 nM, in agreement with previous reports suggesting EZH2 to be a subunit responsible for lncRNA interaction (although these studies did not report a binding affinity).
However, these studies did not examine changes in CREB DNA binding and did not identify features separating cAMP-inducible and non-inducible CREB targets.
The remaining RNA-binding proteins newly identified in this study do not have domains known to bind nucleic acids.
For example, a large scale Y2H analysis of PDZ domains suggested that many PDZ domains do not rely on a free C-terminal region for binding, however the study did not identify the internal binding motifs [ 65].
Therefore the STAT1 binding motif obtained in the present study does not take into account putative low affinity sites.
The C allele creates an additional Sp1-type (CCAC C box) promoter site, and although it was initially suggested to increase expression of the gene [ 9], a subsequent study did not observe binding to the human transcription factor Sp-1 [ 16].
Furthermore, FST315 and FST288 have been shown to bind to other TGF-beta superfamily member proteins, such as BMP-6 and MBP-7 (Sidis et al. 2006), and current study did not examine the binding of FST-type proteins to other TGB-beta superfamily member proteins.
