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Metal binding studies demonstrate that YjiA can bind stoichiometric cobalt, 2 equiv of nickel, or four zinc ions in solution, and loading the protein with metal inhibits the GTPase activity.
Competition binding studies demonstrate that tumour cell beta-adrenergic receptors have both high- and low-affinity agonist binding but are functionally uncoupled from Gs.
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Preliminary binding studies demonstrated that SR31747A also binds to sigma2, a protein that has not yet been cloned, but which is considered as a potential marker of the proliferative status of tumour cells.
In vitro binding studies demonstrated that an Ebola virus surface glycoprotein binds to luminal loop 2 of NPC1 (Miller et al., 2012), the same loop that binds to NPC2 (amino acids 373 620).
Lipid binding studies demonstrated that TGD1 specifically bound 1,2-diacyl sn-glycerol 3-phosphate (phosphatidic acid).
Competition binding studies demonstrated that ondansetron also has high 5-HT3 receptor binding affinity, with a Ki value of 0.47 ± 0.14 nM.
Importantly, in vitro (EMSA) and in vivo (ChIP-seq) binding studies demonstrated that USF1 and CLOCK-BMAL1 significantficant DNA-binding specificity.
Previous native gel-based binding studies demonstrated that only low levels of binding were reported for ISWI and ISWI-containing complexes ACF and ISW2 interacting with nucleosomes containing no flanking DNA.
2 3 Receptor binding studies demonstrated that different types of insulin analogues exhibit different affinities for the IGF-1 receptor compared with native human insulin, and studies have reported colorectal, breast and prostate cell lines to proliferate in response to exposure to some types of insulin analogues, but not to human insulin.
An in vitro binding study demonstrated that TJ-54 showed agonistic binding to 5-HT1A andopaminene (DA) 2 receptors.
Filter-binding studies demonstrated that the addition of Mg2+ inhibits TRAP binding, which may be partially due to the effect of Mg2+ on RNA tertiary structure formation.
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