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The theoretical results are in agreement with experimental binding studies based on laser confocal microscopy, magneto-actuated immunoassay and electrochemical sensing.
Because NMR analysis did not unambiguously reveal which of the arginine residues (Arg3 or Arg4) participates in receptor binding, studies based on targeted phage alanine scanning and IL-11 site-directed mutagenesis were indicated.
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Three of these sites (+57, +53 and +40) were also confirmed experimentally or predicted in other studies based on the consensus binding motif of CTCF [36].
Similar studies, based on the formalism presented here, have been successfully used to evaluate the binding properties of nanoparticles to the biological macromolecules [21, 22].
Protein A was considered in this study based on its efficient binding to wide range of immunoglobulins, and the easy elution of these antibodies by mild chromatographic conditions [37].
A recent study based on genome-wide binding of PML RAR α using ChIP-sequencing in a PML RAR α inducible cell model further shed light on the PU.1 and PML RAR α interaction (Wang et al, 2010).
Herein, we have performed a study based on chromatin immunoprecipitation (ChIP) from living cancer cells to analyze HMGA2 binding sites.
The above study, based on state occupancies, was useful in distinguishing between true positive and false positive binding funnels.
The main aim of this study is to provide understanding for the interaction modes and the binding affinity based on the study of PEG 400 that binds to ctDNA.
Although it is possible that these two related proteins have unique modes of interactions and binding partners, based on our study we raise the possibility that certain key components, such as GAGs and/or metal ions, may be missing from functional assays of myocilin attempted to date.
Genome-wide nucleosome occupancy is negatively related to the average level of transcription factor motif binding based on studies in yeast and several other model organisms.
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