In response to chronic 2-AG overload, compensatory desensitization of brain eCB-CB1R signalling was evident, as both CB1R density (assessed by radioligand-binding studies) and functional responses (assessed by CB1R-dependent G-protein activation assays) were attenuated following chronic MAGL inactivation (Chanda et al. 2010, Schlosburg et al. 2010).
BODIPY-TMR-CGP has previously been characterized at the β2-adrenoceptor in functional and fluorescence imaging binding studies, and unlike the β1-adrenoceptor, only one binding site has been described at the β2-adrenoceptor (Baker et al., 2003b).
Relevant assays should include binding studies, functional studies, and studies of biological activities.
Such a divergence could be related to the differences between the ELISA-binding study and the functional adhesion study due to, incubation times (1 hour +4 hours for binding; 30 minutes +1 hour for adhesion), biological system (only proteins for binding; cells and proteins for adhesion) and sensitivity of the revelation method (HRP for binding; MTT for adhesion).
Surface sensitive optical techniques based on surface plasmon resonance have become interesting for biosciences in the context of biorecognition and binding studies at functional surfaces.
Potencies were similar (r = 0.94) in functional and binding studies, with BUF and ranakinin being most potent.
In the present study we analyzed A2A and A2B AR subtypes in neutrophils from patients affected by SSc by means of expression analysis, radioligand binding assays and functional studies.
In the present study we analyzed A2A and A2B AR subtypes in neutrophils of patients affected by SSc, by means of Western blot, radioligand binding techniques and functional studies.
In summary, the here presented approach of employing TBHP as oxidizing reagent combined with quantitative LC-MS and binding studies greatly facilitates the efficient identification and functional evaluation of methionine and tryptophan oxidation sites in the CDR of recombinant antibodies.
In addition, fluorescent ligands can be used in functional and ligand binding studies in single cell and cell population assays with both primary and recombinant cells (Baker et al., 2003b; Hara et al., 2009; May et al., 2010; Stoddart et al., 2012).
