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The probe-target binding strength is affected by competing formation of probe-probe dimers and secondary structures in probes, resulting in background noise from which low hybridisation intensity signals can be difficult to distinguish [ 5, 8].
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Strength is proportionally related to length, but strength is affected much more by changes in thickness".
Concrete's strength is affected by environmental factors, especially temperature and moisture.
The binding strength is determined by the binding free energy, Δ G bind.
If Ft and Ds are both phosphorylated, binding strength is intermediate.
The binding strength was significantly weakened by carboxymethylation.
The affect of salt on the binding of F-σ32 to Tb-core RNAP was confirmed; the binding was very similar with NaCl or KGlu and the binding strength was not greatly weakened, even at 500 mM.
Hence, larger variations of binding strength are necessary to achieve selectivity.
Ribosome binding strength was estimated by applying two long established facts.
The binding strength was measured using a nano Isothermal Titration Calorimeter (ITC TATA instruments, Newcastle, UK).
The binding strength of the regulators is affected by these variations.
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