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The same ADP binding stoichiometry was also found for the repressed and hyperactive variants.
But, higher binding stoichiometry was also abundant in the cells expressing both eGFP-CaM and the nonlabeled form of αCaMKII, presumably because αCaMKII is binding to multiple copies of eGFP-CaM.
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The methods of data analysis to derive the equilibrium constant and binding stoichiometry are also discussed in the chapter.
Intriguingly, a very similar model of DISC stoichiometry was also reported for the CD95-system.
Evaluation of binding to purified enzymes by surface plasmon resonance (measuring on- and off-rates and affinity constants) is the major high through-put tool but isothermal titration calorimetry (ITC, measuring binding affinity and stoichiometry) is also used.
The binding stoichiometry was determined at peptide concentrations between 1 µM and 10 µM, while the binding constants were determined at a peptide concentration of 1 µM.
To confirm that this phase is associated with a single nucleotide site, its PAPS binding stoichiometry was determined.
Influence from the film stoichiometry is also shortly commented.
Complexes of calcimycin with Mg2+, Ni2+, and Zn2+ cations on 2 : 1 stoichiometry are also described [ 20– 20].
Nevertheless, the result is consistent with the idea that the AtCaM6 W980 binding stoichiometry is 2 1.
Both 1 1 and 2 1 binding stoichiometries were observed for TMPyP4 binding to two types of G-quadruplexes.
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