Besides, spectrometric methods were simultaneously used to verify the interactions and binding stoichiometry between these molecules and GHC.
The binding stoichiometry between HL and M3+ was determined from the Job's plot (fluorescent spectrum) and ESI MS spectrum data to be 1 1.
The 1 1 binding stoichiometry between HL and Al3+ was corroborated by the Job plot and the ESI-MS spectrum.
The binding stoichiometry between HL and Al3+ was determined as 1 1 by the Job's plot.
The binding stoichiometry between sensor and anions was found to be 1 2.
The binding stoichiometry between Cu2+ and L has been investigated using Job's plot and MALDI-TOF mass analysis.
Results were nearly identical for the biotin streptavidin system and the oriented immobilization system, matching the calculated binding stoichiometry between mouse IgG and anti-mouse IgG antibody.
Based on the observed binding stoichiometry between GFP-Lpd850−1250aa and VASP1−114aa, we hypothesize that steric hindrance allows only a single EVH1 domain to interact with the tandem FPPPP motifs (i.e., SPD FPPPPP ESSLV FPPPPP SPVPA and SVVE FPSPP SDSD FPPPPP ETD).
The probe L exhibited a more than 40-fold fluorescence quenching in the presence of Ni2+ with a 1 1 binding stoichiometry between probe L and Ni2+.
