Sentence examples for binding steps in the from inspiring English sources

NC Membranes were blocked for 4 h with 5% nonfat dry milk solution in Tris-buffered saline and as thorough removal of the unbound reagents after each of the binding steps in the procedure.

In addition, FNLDH shows significant positive homotropic cooperativity also for NADH binding, which is less affected by FBP or Mg2+ than pyruvate binding (Table 3), suggesting that FNLDH changes in its structure through two steps, the NADH binding and following pyruvate binding steps, in the allosteric transition.

These results implicate the adoption of the shifted structure of stem-loop I as an important process at the binding step in the VS ribozyme reaction pathway.

DNaseI (100 U/ml) or ethidium bromide (EtBr) (50 µg/ml) was included in the incubation with Rad51 in the binding step in the corresponding wells to test for DNA mediated protein interaction.

The 12 μs (74 μM O2) lifetime assigned to the O2 binding step in the G232V mutant corresponds to a second-order rate constant of ∼1 × 10 M–10s–10

The fluorescence curves were well fit by single exponentials, and the resulting rate constants, kobs, increased with ADP concentration in an approximately linear manner (3, panel b), indicating that the rate of the signal change is mainly controlled by the bimolecular binding step in the concentration range examined.

In this manuscript, Rawling et al. investigated the importance of ATP binding step in RIG-I activation, using biochemical and cell-based approaches.

The society claims that the government missed one crucial – and legally binding step – in its preparations for cuts: that it failed to carry out an "equality impact assessment" to show how the plans will affect women.

In comparison with cis-splicing, trans-splicing has one additional binding step, in which two fragments of split inteins recognize and associate with each other to reconstitute splicing activity, followed by the four steps in protein splicing mechanism [ 2].

Thorough characterization demonstrates that the chimera reports processes that occur in the transport cycle, although at present it is not possible to differentiate substrate binding or steps in the transport cycle.

Branched chain amino-acid, and leucine in particular, enhance muscle protein synthesis through the activation of the mRNA-binding step in translation initiation in skeletal muscle, but not in the liver (Yoshizawa, 2004).