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Taken together, TUT7 may discriminate between the substrates during the binding step rather than after binding to RNA (Supplementary Fig S3B).
The observed rate constants show a linear dependence on the concentration of GDP, indicating that the speed of the fluorescence response in the concentration range measured is controlled by the bimolecular binding step rather than a conformational change.
Furthermore, the preferential selection of dCTP is believed to be achieved at the nucleotide binding step rather than the incorporation step, and the role of hydrogen bonding between the incoming nucleotide and the side chain of R375 was shown to be more important for catalytic efficiency than base stacking.
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Together with the data from binding experiments (Fig. 4B), this result indicates that the preference of Dnmt1 for hemimethylated substrates is determined at the covalent complex formation step rather than upon DNA binding.
We found that ≈80% of changes in the total activation energy resulted from changes in the enzyme substrate binding energy, indicating that substrate selectivity in the small pocket is controlled predominantly by the binding step (KM) rather than the catalytic step (kcat).
It can be introduced relatively cheaply, step by step, rather than being rolled out in one big, costly gamble.
It will be tiny steps rather than one giant stride".
It's baby steps rather than social revolution.
Think baby steps rather than a giant leap, advises Kappesser.
"They can start with small steps rather than needing to be large-scale changes.
Examples from this pilot are intended to illustrate the planning steps rather than prescribe management actions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com