Sentence examples for binding step is required from inspiring English sources
Cohesin-DNA complexes in eco1∆ wpl1∆ cells are stably bound to DNA but fail to establish cohesion, indicating a post-DNA binding step is required for cohesion.
Multiple binding steps are required to account for the observed delay and nonexponential character of channel opening.
This permeabilisation step is required because the anti-γ-H2AX antibody binding requires intracellular access to detect the γ-H2AX protein.
Since the microarray surfaces had been pre-treated to minimize unspecific binding of the target antibodies, no blocking step was required prior to incubation.
No formic acid step was required.
No microarray pre-hybridisation step was required.
It is proposed that at least four different steps are required for E. coli K1 invasion into brain endothelial cells: 1) initial binding, 2) tight binding/invasion, 3) host cell cytoskeletal rearrangement, and 4) intercellular trafficking [27].
