Sentence examples for binding step in from inspiring English sources

The society claims that the government missed one crucial – and legally binding step – in its preparations for cuts: that it failed to carry out an "equality impact assessment" to show how the plans will affect women.

These results implicate the adoption of the shifted structure of stem-loop I as an important process at the binding step in the VS ribozyme reaction pathway.

DNaseI (100 U/ml) or ethidium bromide (EtBr) (50 µg/ml) was included in the incubation with Rad51 in the binding step in the corresponding wells to test for DNA mediated protein interaction.

In this manuscript, Rawling et al. investigated the importance of ATP binding step in RIG-I activation, using biochemical and cell-based approaches.

In comparison with cis-splicing, trans-splicing has one additional binding step, in which two fragments of split inteins recognize and associate with each other to reconstitute splicing activity, followed by the four steps in protein splicing mechanism [ 2].

The 12 μs (74 μM O2) lifetime assigned to the O2 binding step in the G232V mutant corresponds to a second-order rate constant of ∼1 × 10 M–10s–10

NC Membranes were blocked for 4 h with 5% nonfat dry milk solution in Tris-buffered saline and as thorough removal of the unbound reagents after each of the binding steps in the procedure.

In addition, FNLDH shows significant positive homotropic cooperativity also for NADH binding, which is less affected by FBP or Mg2+ than pyruvate binding (Table 3), suggesting that FNLDH changes in its structure through two steps, the NADH binding and following pyruvate binding steps, in the allosteric transition.

Branched chain amino-acid, and leucine in particular, enhance muscle protein synthesis through the activation of the mRNA-binding step in translation initiation in skeletal muscle, but not in the liver (Yoshizawa, 2004).

Taken together, these findings suggest that the active site residues forming the small pocket are mainly engaged in the binding step but not significantly involved in the catalytic step, which may provide insights into how to design a rational strategy for engineering of the small pocket to relieve the steric constraint toward bulky substituents.

Whether His292 is unprotonated and phosphite monoprotonated in the binding step, or whether dianionic phosphite binds to PTDH with a protonated His292 is currently not known, but either possibility suggests that the nucleophilic displacement reaction takes place on monoprotonated phosphite.