given a sorted array of continuous values S and a corresponding array of binding status of the target protein, the method finds a best cut point T that partitions the range of S into two non-overlapping intervals.
Each distinct binding status of DNA is associated with a unique transcription initiation rate, and then the overall rate of mRNA synthesis is a weighted average of the initiation rates for distinct binding status, where the weights are given by the relative abundance of each configuration at equilibrium, determined by the calculation of binding energy [ 46].
The binding status of Gαi1 was assayed by a controlled trypsin digestion assay (Mazzoni et al., 1991; Marin et al., 2001) in which the activated form of Gαi1 (GTPγS) is protected from digestion by N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) treated trypsin.
To combine information from the supporting tracks and predict the binding locations of a target protein, we fit a logistic regression model between a vector of binary indicators Y = (Y1, Y2,…, Y n ) denoting the known binding status of the target protein at n probes, and a data matrix X of m supporting tracks.
The nucleotide binding status of Gtr1 is regulated by the Vam6 guanine nucleotide exchange factor, which is a component of the homotypic fusion and vacuole protein sorting complex in which it promotes nucleotide exchange of Ypt7, the yeast homology of mammalian Rab-7.
We compared the varying binding status of SATB1 at the MAR elements in β-globin cluster during the erythroid differentiation.
To answer this question, more than 90% K562 cells were synchronized into G2/M phases (Fig. S5) and ChIP was performed to observe the binding status of SATB1 at MARHS4, MARHS2 and MARε.
This finding indicated that Nup98-Crm1 associstion is dependent on the cargo binding status of Crm1; therefore, we examined whether such properties of Nup98FG were still preserved in Nup98-HoxA9.
Note that the feedback loop is not explicit in Fig. 1 but implicitly included through the dependence of RNAp-promoter binding equilibrium on the binding status of the TF-operator pair.
The non-binding status of the Dear Colleague Letter meant that a new Administration could easily retract it with another letter, much in the same way that the Trump Administration retracted the guidance on transgender students earlier this year.
