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Rags pose a particular experimental challenge for identifying factors that regulate the nucleotide state of a single Rag, as they exist as obligate heterodimers, and so we developed several methods that allowed us to analyze the nucleotide binding state of one Rag at a time.
This might arise either by the binding state of one WW-domain being able to influence the affinity of the other for its target, or through possible different orientations of the two WW-domains in the Nedd4-2 WW2-3 protein compared to its Nedd4 counterpart.
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In contrast, only one stable binding state of Cl is identified in the form of MgCl2 for the TiCl3-doped MgH2.
The site of CO2 binding was demonstrated6,7 to occur at the Val-1β site8 linked to the O2 binding state of the β-chain9.
These binding energies are close to the reported values of the binding state of Zn2+[19].
(C ) Rab6A affects the strong microtubule binding state of KIF1C.
The product distribution for a wide-range of products observed for the geranylation of 1,6-DHN catalyzed by NphB complexed with geranyldiphosphate is partly due to the free energy preferences of the substrate binding states that favor one reaction channel over another.
Each row shows the geometry of the nucleotide-binding pocket of one ATPase in all six states.
Here, we show that the binding of one receptor at the glucocorticoid response element (GRE) does not reduce the steady-state binding of another receptor variant to the same GRE.
Side views of the closed, (left; before binding to TcA) (PDB 4O9X) and open (right; after binding to TcA) state of the TcA-binding six-bladed β-propeller domain of TcB TcC.
Here, the receptors are locked in conformations equivalent to agonist-bound states, and it is observed that each binding site initiates the formation of one of two 'primed' states from which the channels are able to open and close.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com