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These results suggested that salt stress reduced the binding stability of several subunits of PSII.
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Figure 1: Affinity and binding stability of DNA/protein interactions are changed by the methylation of non-CpG context.
Leaching experiments were performed to prove the binding stability of the GFP-doped nanoparticles.
These mutations affect the DNA-binding stability of WT1 to the target gene [ 73].
We report here the use of multiple stabilizing substitutions to increase the stability of p16INK4a and furthermore, to restore Cdk4 binding activity of several defective, cancer-related mutant proteins.
In vitro binding and melting studies have shown, for short duplex DNA oligomers of 12 to 22 base pairs, that thermodynamic stability of flanking sequences is inversely related to the binding strength of several DNA restriction enzymes and site-specific binding ligands.
Therefore, changes of binding stability (koff) are expected to have a strong influence on gene expression level.
High-accuracy mapping of protein binding stability on nucleosomal DNA using a single-molecule method.
The binding stability was used for analysis of false-negative SFPs.
Recent studies suggest that the efficacy of such off-targeting might be dependent on binding stability.
Here, we increase the regulatory range of the Rnt1p control elements, by modifying a critical region for enzyme binding to its hairpin substrates, the binding stability box (BSB).
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