Sentence examples for binding specimens were incubated from inspiring English sources

To detect scFv binding, specimens were incubated for 1 h with streptavidin/horseradish peroxidase conjugate (1 : 50).

After treatment with 3% BSA for 30 min to block non-specific protein binding, a primary antibody specific for MKP-1 was applied (dilution: 1 : 100), and the specimens were incubated overnight.

Twenty-five MDC and/or SIS specimens were incubated at 37°C for either 10 or 20 days.

Specimens were incubated at 37°C for an hour with gentle rocking.

Specimens were incubated with Ki-67 MAb (1 100; DAKO, Carpinteria, CA, USA) overnight at 4°C.

The bone specimens were kept in the freezer while the catheter specimens were incubated for 7 days under aerobic conditions, at 35°C.

After washing, specimens were incubated with the appropriate primary antibody overnight at room temperature (RT).

As a negative control specimens were incubated without the primary antibody.

Specimens were incubated at 37°C and examined on a weekly basis for eight weeks.

After washing twice in PBS, specimens were incubated with appropriate secondary antibodies conjugated to Cy2 or Texas Red.

The specimens were incubated with the primary antibodies diluted in PBS 0.1M containing 0.04% triton overnight at 4°C.