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Additional confirmation of binding specificity was obtained from competition experiments in which the wild-type, but not a mutated, SL3 oligonucleotide effectively competed with probe for L22 binding (compare 100× SL3 with 100× mSL3, Fig. 3B).
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Another alternative approach is pretargeting, whereby tumour target antigen binding specificity is obtained by injecting unlabelled antibody derivatives followed by a radiolabelled low molecular weight compound that specifically binds to the tumour and is rapidly cleared from the circulation (Goodwin et al. 1986).
However, limited specificity was obtained (57%) [92].
After 14 rounds positive selection and 4 rounds negative selection, two aptamers with high binding affinity and good specificity were obtained, which have the dissociation constant Kd values of 1.60 ± 0.16 μM and 0.76 ± 0.18 μM, respectively.
Increased sensitivity and specificity were obtained from real-time PCR.
RBP sequence specificities were obtained by our motif discovery approach.
High sensitivities and specificities were obtained when diagnosing meniscal rupture.
We obtained 14 different peptide aptamers, whose binding specificity was determined using a yeast two-hybrid mating assay.
Similar binding specificity was shown for the parent antibody V5B2 [13].
Specific binding was obtained by subtracting non-specific binding from total binding.
The (4S -FPro turned out to be a DNA binder with lower specificity; comparable binding was obtained for 3,4S -FProoproline witurnedlanar ring coutormatoon.
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