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The surface of a gold electrode was modified by AP site-containing DNA duplex on which Ag+ binding specificity was evaluated.
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To evaluate a protein-protein interaction, a protein complex score is calculated as (13) E (s 1, c 1, s 2, c 2 ) = ∑ i, j U a b (i, j ) C (i, j ) where C is evaluated between c1 and c2 (inter-molecularly), and the sum runs over all residue pairs (i, j) such that i comes from c 1 and j comes from c 2. Folding and binding specificity were evaluated with several considerations.
Assay specificity was evaluated by eliminating InO coating of wells.
Antibody binding activity and specificity were evaluated by ELISA.
The binding specificity was also evaluated by measuring the uptake of 111In-DTPA-11B6 in hK2-negative DU145 xenografts expressing low levels of hK2 (n = 3) at 48 h post-injection, which are considered to be hK2-negative when compared to other PCa tumours.
Their DNA-binding affinities and sequence specificities were evaluated by SPR and Bind-n-Seq analyses.
Actin binding specificity was demonstrated by competitive binding experiments and comparative staining of well-known structures.
Similar binding specificity was shown for the parent antibody V5B2 [13].
The binding specificity to hK2 was evaluated by both in vivo competitive binding assays with excess non-labelled 11B6 and hK2-negative DU145 xenografts.
(b) Specificity of CCK2R binding was evaluated after blocking with increasing amounts of non-fluorescent CCK2R ligand.
Details of binding specificity are provided in SI Figures S5 S7.
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