Sentence examples for binding specificity was determined from inspiring English sources
Cytokine binding specificity was determined using a BIAcore X biosensor (Biacore, Uppsala, Sweden).
We obtained 14 different peptide aptamers, whose binding specificity was determined using a yeast two-hybrid mating assay.
Binding specificity was determined either by pre-treating the BeWo cells with 0.5 units/ml of chondroitinase ABC (Fluka) or 25 units/ml Streptomyces hyaluronlyticus hyaluronidase (Calbiochem) or by pre-incubating the cells with 5 µg/ml anti-CD36 monoclonal antibody FA6/152 (Beckman Coulter) or 5 µg/ml anti-ICAM-1 monoclonal antibody 84H10 (Beckman Coulter) for 1 h at 37°C.
Binding specificity was determined by evaluating radioactive displacement.
The binding specificity is determined jointly by a 20-bp matching region on the sgRNA and a short DNA motif (protospacer adjacent motif or PAM, sequence: NRG, R = G or A) juxtaposed to the DNA complementary region.
The major advantage of this system is that only one dCas9 activator is required to activate multiple genes individually or simultaneously and that its DNA binding specificity is determined by sgRNAs, which are designed based on simple RNA/DNA complementarity.
Experiments of mutant proteins demonstrated that binding specificity is determined by the core basic region and the hinge region independently, and the two regions have an additive effect on DNA-binding specificity.
The targeted DNA binding specificity is determined by TALE repeats through protein DNA contact in TALEN-mediated genetic modifications, whereas in the case of a CRISPR/Cas9 system, the specificity is determined by the gRNA binding through RNA DNA contact (Miller et al., 2011; Zhang et al., 2011; Jinek et al., 2012).
Nucleotide-binding specificity is determined not only by possible contacts with nucleotides but also by steric exclusion of interactions with alternative nucleotides (reviewed in [ 25]).
Binding affinity and specificity was determined using direct binding assays with toxoid-coated and toxin-coated microspheres as described in the methods.
