Sentence examples for binding specificity was carried from inspiring English sources
Determination of the binding specificity was carried out by preincubating cells with increasing concentrations of unlabelled peptide (HG-13 Minigastrin, Bachem, Weil am Rhein, Germany) ranging from 0.1 to 2,000 nM directly before adding a fixed probe concentration of 2 nM to all samples.
For hiomt in situs, probe binding specificity was improved by carrying hybridization and post-hybridization washes at 62 ° C and by using a lower SSC concentration for the last post-hybridization washes (0.1x SSC instead of 0.2x SSC).
Similar binding specificity was shown for the parent antibody V5B2 [13].
The link between determining binding specificity and finding sites where the transcription factor is likely to bind is the way in which binding specificity is represented.
Details of binding specificity are provided in SI Figures S5 S7.
The network is constructed by connecting genes with binding sites if their binding specificity is equal.
Standard PCR was carried out to determine the specificity of AP-2γ binding DNA fragments derived from the AP-2γ-ChIP and normal rabbit IgG negative control.
For each method, a 10-fold cross validation was carried out to assess the performance in predicting binding specificity for test DNA sequences.
To demonstrate the specificity of the Ab3 response against anti-Id scFv, an inhibition ELISA was carried out by inhibiting the binding of each anti-Id scFv on trastuzumab F ab′ 2 fragments by serial dilutions of sera from immunised mice.
Independent analysis of the mouse ETS family was carried out using PBMs, which allow determination of TF-binding specificities through sequence-specific binding of individual TFs directly to double-stranded DNA microarrays containing all possible 10-mer binding sites (Berger et al, 2006).
