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Binding specificity was assessed by preincubating cells with the nonfluorescent A3AR antagonist MRS 1220 (100 nM) for 30 min at 37°C before the addition of CA200645.
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Sequence specificity was assessed by comparing binding signals for a panel of methylated or unmodified peptides within the library.
Receptor specificity was assessed with unlabeled exendin-3.
Amplification specificity was assessed by melting curve analyses.
Immunoreactive specificity was assessed by incubation with blocking peptide.
Immunoreactive specificity was assessed by omitting the first antibodies.
Antibody specificity was assessed by Western blotting.
The specificity was assessed using melting curves.
Primer specificity was assessed by melting curve analysis.
Amplification specificity was assessed with a melting curve analysis.
The primer specificity was assessed by using the BLAST software.
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