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Immunologically, hnRNP A1's nucleocytoplasmic shuttling and RNA binding specificity is required for myelopoiesis and modulation of immune-mediated programmed cell death.
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Thus truly unbiased, genome-wide methods capable of preserving the in situ RNA binding specificity are required to elucidate the functions of RNA binding proteins in an efficient and comprehensive manner.
High primer specificity is required in non-defined samples.
Trypsin specificity was required for all peptides.
Together with previous studies, our data suggest that at least three N-acetylglucosaminyl transferases with different specificities are required for synthesis of the ligand-binding glycan on αDG.
Enzymes with different specificities are required to hydrolyze these residues.
The major advantage of this system is that only one dCas9 activator is required to activate multiple genes individually or simultaneously and that its DNA binding specificity is determined by sgRNAs, which are designed based on simple RNA/DNA complementarity.
The link between determining binding specificity and finding sites where the transcription factor is likely to bind is the way in which binding specificity is represented.
The network is constructed by connecting genes with binding sites if their binding specificity is equal.
Similar binding specificity was shown for the parent antibody V5B2 [13].
Details of binding specificity are provided in SI Figures S5 S7.
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