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However, the ATGA binding specificity is lost when the two thiophenes are replaced with two furans (DB914), and related compounds DB832, DB934, DB1246, DB1324, DB1579, DB1315, and DB1256, or by two selenophanes (DB1282 and DB1273 with phenyls or pyridines, respectively).
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These activities of ataxin-3 are UIM-dependent, as high affinity ubiquitin binding and cleavage specificity are lost when all three UIMs are mutated [16].
The link between determining binding specificity and finding sites where the transcription factor is likely to bind is the way in which binding specificity is represented.
Overall, we suggest that from an ancestral protein with high affinity but low specificity toward Cbl, the increase in binding specificity was acquired (and lost) through gene duplication and recurrent mutations at the carrier Cbl binding pocket (fig. 3 and table 1).
Actin binding specificity was demonstrated by competitive binding experiments and comparative staining of well-known structures.
Similar binding specificity was shown for the parent antibody V5B2 [13].
Details of binding specificity are provided in SI Figures S5 S7.
Also CAP binding is lost.
Interestingly, this binding is lost in H19 Δ3/+ mol MEF.
So, what is gained in specificity for microarray oligo-design is lost for gene annotation.
Something is lost nonetheless in playing down the tale's Jewish specificity.
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