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Appropriate tracers have a great binding specificity for molecular targets, upregulated under specific pathologic settings, permitting comparison to the physiological state.
As a pair, the central repeat regions of TALENs determine the DNA binding specificity for the two sub-target sites; and the dimeric non-specific FokI cleavage domains cause a DNA double strand break (DSB) between the bound sequences.
The binding specificity for MC-1 was assessed by pre-administration of rotenone, a specific MC-1 inhibitor.
Plant bZIP proteins harbor a relaxed binding specificity for DNA sequence motifs containing an ACGT core, and preferentially bind to the G-box (CACGTG), C-box (GACGTC) and A-box (TACGTA) [ 3].
It shows the high binding specificity for glyphosate (K D = 8.6 μM).
Interestingly, the peptides with high ZnO affinity obtained exhibited binding specificity for ZnO.
We used the computational protein design approach to modify CaM binding specificity for these two targets.
A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose containing glycans.
The conjugate retained its binding specificity for SW1116 cell with a similar activity with KM231.
Interestingly, cyG7A5 showed a higher binding specificity for IgG than did Protein A.
The genetically engineered anti-CD20 scFv and GPI-tagged derivative have binding specificity for the CD20 antigen.
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