The aa-sequences of fungal LysM domains are highly variable, and they could therefore be important for substrate-binding specificity and, thus, targeting of the chitinase to its correct place for hydrolysis.
Regardless of the data source, binding site prediction typically requires some information about binding specificities and is therefore dependent on previous analysis.
It is therefore likely that Kap121 binding specificity and cargo selectivity are determined by several mechanisms.
The link between determining binding specificity and finding sites where the transcription factor is likely to bind is the way in which binding specificity is represented.
In vitro binding and specificity were assessed for 125I-Fab, and binding, specificity and internalization were assessed for 111In-Fab using both H314 and A431 cells.
Here, we report a unique binding specificity and mode of ligand binding for a family 32 CBM.
Natural selection might promote heterodimers via the increase of the combinatory potential of the OBP (increasing either the spectrum of possible target odorants or the binding-specificity) and maintaining, therefore, the co-localization of different OBPs in the same cells.
However, there is very limited information about the key binding partners underlying binding specificities and key differences for the two domains.
Therefore, these scDR1αβ molecules will provide a powerful technology platform for further design of DR1 molecules with improved peptide-binding specificity and affinity for therapeutic and diagnostic applications.
