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It is therefore likely that Kap121 binding specificity and cargo selectivity are determined by several mechanisms.
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In vitro binding and specificity were assessed for 125I-Fab, and binding, specificity and internalization were assessed for 111In-Fab using both H314 and A431 cells.
Motor cargo adaptors not only regulate binding affinities, but also mediate transport specificity and cargo identity, thereby contributing to the regulated delivery of particular cargo to its specific cellular destination (Schlager and Hoogenraad, 2009).
Evaluation of a polyclonal anti-CRP reagent for binding specificity to pCRP and mCRP antigens.
The sequence C-terminally adjacent to KIM, termed kinase-specificity sequences (KISs), provides binding specificity to ERK1/2 and p38.
Evolution of TF binding specificity by gene duplication and divergence.
The advantages of noncovalent binding between CPP and cargo are ease of use, ease of production, versatility with respect to cargo composition, and preservation of cargo functionality [ 27].
Transcription factor binding specificities can also be seen by comparing the Hth and Ubx binding sites.
The preSec23/24 may have possessed both the Sar1-binding/GAP activity of Sec23 and the cargo binding capability of Sec24, suggesting that the preSec23/24 could bind both Sar1 and cargo.
The contrast between in vivo-and in vitro-binding specificity suggests an intrinsic molecular mechanism or the involvement of other proteins for the specific compartmentalization of DLCs and their pro-apoptotic cargoes.
Multiple paralogs, each with multiple binding sites, would have drastically increased the number or specificity of cargo binding, and therefore many have been selected for.
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