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Our method detected novel binding specificities for three RBPs for which previous motif finding attempts had failed.
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The DREAM4 PDZ-peptide recognition challenge consisted of predicting binding specificities for five multi-mutant ERBB2IP-1 sequences (Figure 1).
In the general case, deletion between two different, asymmetric sites might require up to four ZFRs, each with DNA-binding specificity for one half-site.
As a pair, the central repeat regions of TALENs determine the DNA binding specificity for the two sub-target sites; and the dimeric non-specific FokI cleavage domains cause a DNA double strand break (DSB) between the bound sequences.
We used the computational protein design approach to modify CaM binding specificity for these two targets.
This gives the antibody bispecificity, allowing for the binding specificities of two different antigens.
We obtained binding specificities for 112 DNA-binding proteins representing 19 distinct structural classes.
Residue 31 in Rap and Ras is a key residue for determining the binding specificity for downstream effectors of these two closely related small GTPases (Nassar et al., 1996).
These differences arise from changes in three major physicochemical parameters: binding specificity for complex carbohydrates, the pH-dependent oligomerization state (dimer-tetramer equilibrium), and the relative orientation of the carbohydrate-binding sites.
The binding specificity for MC-1 was assessed by pre-administration of rotenone, a specific MC-1 inhibitor.
Within this group were three known methyl-lysine-binding TDs (53BP1, PHF20, and JMJ2A [25] [27]) as well as a TD with binding specificity for non-histone arginine methylated substrates (SMN [26], [28]).
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