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Transcriptome profiling of 55 overexpression strains with reduced fitness identified putative target genes and binding specificities for most known and several uncharacterized transcription factors (Chua et al. 2006).
The DNA binding specificities for most ApiAP2 in Plasmodium falciparum have already been described [ 33] and a similar effort for the T. gondii ApiAP2 binding specificities is nearly complete (Ranjan et al., in preparation).
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Likewise, the binding specificities of most of the putative C. parvum transcription factors are not known, particularly the many zinc finger and ApiAP2 proteins; these unknown motifs could represent binding sites for these transcription factor proteins.
We obtained binding specificities for 112 DNA-binding proteins representing 19 distinct structural classes.
The most common binding specificity for the PX domain appears to be for PI(3 P; hence, several PX domain-containing proteins localize to PI(3 P-rich endosomal and vacuolar structures through this domain.
Although most substitutions will likely disrupt binding specificity for the current target site, a few may create new interaction sites leading to the emergence of novel functions.
Custom reverse transcriptase (RT) primers for detection of each miRNA, with a stem-loop sequence to enhance the binding specificity for mature miRNA, were designed by Applied Biosystems (Foster City, CA, USA) against the most common isoform.
Whaley, S. et al. A. M. Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly.
Whaley, S. R., English, D. S., Hu, E. L., Barbara, P. F. & Belcher, A. M. Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly.
Interestingly, the peptides with high ZnO affinity obtained exhibited binding specificity for ZnO.
It shows the high binding specificity for glyphosate (K D = 8.6 μM).
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