Sentence examples for binding solution was added from inspiring English sources
The medium was then removed from the cells and 100 mL of 1X dye binding solution was added and plates were incubated for one hour at 37°C.
Briefly, the cell growth medium was removed and 100 µl of 1x lysis/dye binding solution was added into each well.
After incubation, 400 μl binding solution was added to each tube and fluorescence was detected using flow cytometer (BD Biosciences).
Subsequently 150 µl of 1x Annexin-V antibodies in binding solution were added to each well, followed by 15 min incubation.
The solutions i.e. QC samples, CC standards and unknown plasma samples, were freshly prepared as; each sample (600 µl aliquot) alongwith a 50 µl IS (50 ng/ml) was taken in a glass tub, 5% formic acid (200 µl) solution was added (breaking protein binding) and vortexed (300 rpm, 5 min).
After mixing with 240 μl of NaClO4 binding buffer, 60 μl of glass milk solution was added and the suspension was gently shaken at room temperature for 10 min then centrifuged at 7,000 × g for 30 s.
Then Gd3+ (as Gd3+ in solution) was added to preformed lipid nanoparticles (d = 60 70 nm) expressing DTPA-PE (for binding to Gd3+ as Gd-DTPA-PE chelate).
After 10 minutes shell solution was added.
This solution was added to 2.5 ml Ringer's solution.
After washing, a substrate solution was added.
