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If ZNF263 has maintained its repressive role as a KZNF, hypomethylation at its binding sites would predict a repressive effect on the regulation of SINE-linked genes that are normally highly expressed (such as many housekeeping genes).
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Identification of TF binding motifs followed by bioinformatics screenings of possible binding sites would be necessary to predict downstream targets more accurately.
Since the KD describing N-HSP72 binding reactions is 1.0 μM, significant RNP-HSP72 interaction would be predicted within the cell, although saturation of N protein binding sites would be below 50%.
A protein binding site would be an attractive hypothesis.
The rates are not as high as a diffusion-controlled binding mechanism would predict, 108 109 M−108 109.
Magic nuclei are more stable than the binding energy equation would predict.
We further examined how substitutions of amino acids around the predicted ATP-binding site would influence the biochemical properties of the ERT-mt6 RT.
Taken together with the coplanar arrangement of all the ligand-binding sites, from which we would predict effects subsequently confirmed by our mutagenesis data, it seems reasonable to equate our open structure with the "activated" form of AP2 on the plasma membrane.
Docking simulations predicted that the ATP-binding site would be similar between NRTI-sensitive and -resistant RTs during DNA polymerization.
SBVS requires structures of target binding sites to predict potential interactions with ligand molecules.
Because the different binding region of a SAHA analogue may interact differently within the enzyme's active site pocket, one would predict that the binding free energies of various SAHA analogues would be substantially different (see Table 1).
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