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A more comprehensive picture of potential 14-3-3 14-3-3 14-3-3 would help to define how the complete 14-3-3-interactome system works.
This high abundance of the non-canonical binding sites would help offset the low occupancy of individual non-canonical sites, such that at any moment more than half of the bound miRNA might reside at non-canonical sites, yielding more non-canonical than canonical sites when using experimental approaches with such high specificity that they can identify a site with only a single read.
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More robust sampling over a larger number of collection sites would help in confirming this trend.
However, I feel that showing a multiple sequence alignment of the RNase H family highlighting the members used in this study as well as the positions that constitute the substrate and metal binding sites would considerably help understand that indeed this pre-organization comes about despite divergent binding-site sequences.
In different tissues, with distinct chromatin accessibility profiles, different binding sites would be occupied.
The binding sites would not only reveal mRNA targets but would show GQ motif consensus if that's really the in vivo binding site for Aven.
A defined consensus sequence for the AtoC binding site would greatly facilitate the identification of potential AtoC binding elements.
The elucidation of the aminoglycoside-binding receptor would help design a strategy to prevent against aminoglycoside-induced nephrotoxicity.
A glycosylation site adjacent to receptor binding sites may help increase virus infectivity in host cells (7 ).
Until we have this experimental data, mathematical models of binding sites will help us predict TFBSs and in turn help us infer regulatory effects.
Using the same amplifying strategy as before the H2-binding site would also be present offering a second binding site.
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