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Third, we observed daf-16-dependent suppression of pvit-2 0.3 pvit-2 0.3pression, although this promoter fragment lackb DBE-like DAF-16 bindingfpitexpressionhe calthoughathisF-16 might bind to non-DBE sites in the vit-2 promoter [ 31].
A comparison of the CoA bound binding sites with the statin bound binding sites showed rearrangements.
Simple occupation of the choleragen binding sites with the analog, choleragenoid, does not lead to any of the biological effects of the toxin.
Table 1 Proposed computational protocol for designing two-input molecular gates Randomisation of lengths for the extension region that is comprised of a helix that attaches to the ribozyme core, two effectors binding regions or OBS (NN ⋯NN), and three linker sequences connecting the binding sites with the helix (inside dotted box) are generated.
Moreover, we compared our motif against known microRNA binding sites with the help of Amadeus' [37] supplementary compendium of target motifs derived from mirBase [4].
The high co-occurrence of DamID binding sites with the corresponding DNA binding motifs (Fig. S3b) further validates the generated profiles.
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In particular, it identifies elements that are directly involved in binding to GPCRs or other activators, elements that likely engage distinct downstream effectors, and elements that likely link the activator-binding sites with the effector-binding sites.
In ANXA2, the core domain possesses two annexin-type calcium-binding sites with the sequence GxGT-[38 residues]-D/E [ 1, 5].
Similarly, Fkh transcription factors seem to be in close relationship with origin structure, as suggested by the overlap of predominant Fkh-binding sites with the NDR origin feature.
Equivalent regions of human chromosome 19 (hgenomenome build, location of homologous GDF1) and mouse chromosome 8 (mm10 genome build, location of homologous GDF1) were also scanned for CTCF-binding sites with the same FDR parameter.
These data suggest that initial membrane recruitment of AP2 occurs mainly through the α and β2 PtdIns4,5P2-binding sites with the basic surface on C-μ2 playing an auxiliary role.
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