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Thus at least for miRNA-mRNA binding sites with perfect WC complementarity in a 7-nt seed region, dinucleotide frequency bias is a main contributor to stability of the predicted duplex.
We show that many energetically favourable binding sites with perfect seed matches, i.e. potential miRNA targets, occur in all mRNA regions, and that a substantial number of these sites are reassigned between mRNA regions depending on the specific transcript isoform.
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Most of predicted miRNAs have at least one binding site with perfect complimentarity to seed region.
The three synthetic double-stranded DNA targets were used as targets (PM target: four probe-binding sites with perfect match, Del: four probe-binding sites with single deletion, Ins target: four probe-binging sites with single insertion) (Additional file 2: Table S1).
The reason that hAFP did not evolve a large flat ice-binding site with perfect ice lattice match may be due to the involvement of the Ca2+ cofactor, which provides sufficient binding force through coordination of water in the ice lattice.
The number of high-quality potential binding sites with a 6 7 nt region of perfect complementarity at their 5' end (i.e. a seed region) is substantially (1.34 1.87 times) greater than expected under the null model reflected in the MS control set, and slightly (1.06 1.33 times) greater than expected under the null model reflected by FOM.
KLF4 and Sp5 binding was observed at both esBAF and GBAF binding sites, with slightly greater occupancy at GBAF complex binding sites.
Van Nostrand, E. L. et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP).
Comparison of these proteins' in vitro binding sites with their in vivo binding sites indicates that PBM-derived specificitiesificanies caccuratelyely reflect in vivo DNA sequence specificities.
An algorithm for finding protein DNA binding sites with applications to chromatin-immunoprecipitation microarray experiments.
A comparison of the CoA bound binding sites with the statin bound binding sites showed rearrangements.
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