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CCAAT/enhancer binding proteins (C/EBP) include multiple family members that bind to CEBP binding sites with different affinities; that may influence the precision of the present prediction.
TFs bind to a broad spectrum of binding sites with different affinity and change targets widely among species.
Another important factor that may contribute to the variability of radioligand binding to TSPO is the presence of several binding sites with different affinities dependent on single nucleotide polymorphism of TSPO, at Ala147Thr [26].
The multiple-binding-site hypothesis was tested with the Virtual Cell software, assigning to NS1-deYFP two distinct and immobile binding sites with different affinities.
PRRII sequence contains three AT-tracts, separated by 6 and 7 bp, the longest one being 19 bp long, corresponding to three potential binding sites with different affinities for HMGA1.
The precise reason for this observed biphasic kinetics for fatty acid cannot be discerned at present, although, several plausible reasons for such kinetic behavior could involve the presence of multiple substrate binding sites with different binding affinities or binding of substrate in more than one orientation with different affinities [44], [45].
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For either process, the molecule should bear more than one binding site, with different affinities, to drive the motion of the ion.
Interestingly, tasiARF1 and tasiARF3 degraded the same target (AFR3, 002399 m) at the same cleavage site (the 11-th nucleotide from the 5′ end of the small-RNA binding site) with different cleavage efficiency (i.e., approximately by a 6 to 1 ratio) under the CS condition (Table 4, Figure 3A and 3B).
As a result, at least three putative PS-binding sites with different affinities to PS molecule were delineated.
3 Kb regions spanning upstream of the transcription start sites of 91 genes with altered transcript levels and 137 control genes (genes on microarrays, with least possibility of being differentially expressed) were extracted and searched for the presence of full NFI-binding sites, with different stringencies.
ChIP-qPCR validations were carried out on 15 Klf5-binding sites with different numbers of ChIP tag counts within the defined overlap region (Additional file 4).
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binding patterns with different
binding sites with differential
binding TFs with different
binding sites with relevant
binding sites with five-fold
binding sites with perfect
binding sites with high-resolution
binding sites with apparent
binding surfaces with different
binding data with different
binding strengths with different
binding sites with nm
binding regions with different
binding sites with weak
binding peptides with different
binding studies with different
binding sites with conserved
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