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Binding studies with a radiolabeled ligand revealed 710 fmol/mg binding sites, which is about fivefold higher than that of wild-type levels (150 fmol/mg for mock-transfected HeLa cells).
On GABP dataset, we found that most of the binding regions have two or more adjacent binding sites, which is consistent with the literature that GABP binds in a more distributed manner [ 33].
The structure of this model allows for variable spacing between functional binding sites, which is known to play a key role in the transcription initiation process.
In the case of CA IX inhibition, CA II along with other isoforms of CA provide off-target binding sites which is undesirable for cancer treatment.
This study on amino acid interactive network for different interactions facilitates the identification of binding sites which is strongly linked with protein function, and furthermore provides reasonable understanding of the underlying laws of evolution in genomics and proteomics.
Information on the mechanisms of action and 3D structure of the binding sites, which is required for drug design, is available from the 3D structure of α-neurotoxin complexes with nAChR models.
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In the previous experiments we could identify three lectins with opposing binding sites which are able to crosslink adjacent vesicles.
Subsequently, low-temperature carbonization (500 800 °C) and KOH activation leads to abundant ultrasmall pores and strong oxygen molecule binding sites, which are essential in enhancing the capacitance as well as ORR activity.
These enhancer regions seem to contain transcription factor binding sites which are essential to maintain SOX9 expression during development.
This necessitates using predicted ligand binding sites, which are often less accurate than those extracted from ligand-bound structures.
Amygdala kindled seizures resulted in a 30% reduction of [3H] QNB binding sites which was distributed uniformly across the entire molecular and granule cell layers.
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