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Studies on the mechanism of cleavage by 3-finger ZFNs established that the preferred substrates were paired binding sites, which doubled the size of the target sequence recognition from 9 to 18 bp, long enough to specify a unique genomic locus in plant and mammalian cells.
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In Arabidopsis, for example, a TAS3 gene has a pair of miR390 binding sites, which define and produce a single RNA strand that is subsequently turned into a double stranded RNA by RDR6 polymerase.
The PACAP specific binding sites which were not shared VIP were termed type I, while the binding sites shared with both PACAP and VIP were termed Type II.
To generate a list of unambiguous CTCF negative RAD21 binding sites we removed these sites, leaving 667 reassessed CTCF independent RAD21 binding sites, which we define as motif and CTCF-independent RAD21 binding sites (MCIB).
The sequence of this binding site does not resemble any known ATP binding sites, which have much higher binding affinities.
Sum1 binds a DNA binding site which resembles, and partly overlaps, the binding site of Ndt80 [ 23], [ 18].
Among many other binding sites lost in the –673 construct were an E-box, a STAT binding site which forms a sequence model (Genomatix modelInspector) together with an ets binding site, and a Myc associated zinc finger binding site (MAZF).
Any binding site which had a p < 0.01 was annotated as a potential miRNA binding site.
When binding to DNA, LysR protein forms a homodimer and targets two specific binding sites, a regulatory binding site, which has the conserved T-N11-A/TTA-N7/8-GAA T-N11-A/TTA-N7/8-GAA T-N11-A/TTA-N7/8-GAA T-N11-A/TTA-N7/8-GAA T-N11-A/TTA-N7/8-GAAig. 2b).
Locations included the Hatley Park National Historic Site and Royal Roads University, which doubled for the X-Mansion.
The CRDs exhibit conservative characteristic in structure, and in each CRD there are a characteristic double-loop stabilized by two highly conserved disulfide bridges, and a Ca2+ binding sites 2 which is involved in carbohydrate binding [15].
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