Sentence examples for binding sites were then from inspiring English sources

Hence, 5 μg/mL of recombinant hemagglutinin (HA) protein (Sino Biological Inc., Beijing, China), H3N2 virus, and BSA (control) were coated independently in 96-well microtiter plates, and residual protein binding sites were then blocked with 1% skimmed milk in PBS.

Specific binding was obtained after 4 to 6 cycles; selected binding sites were then cloned into pCR2.1-TOPO vector (Invitrogen) and sequenced.

The six miR-2 binding sites were then removed by digestion with SacII and re-ligation of the remaining backbone, creating the pRL-A62 constructs.

The putative binding sites were then further validated using JASPAR and ChampionChIP databases [22], and carefully selected for ChIP-qPCR analyses.

Non-specific binding sites were then blocked with 4% BSA/TBST (w/v) for 10 min. The cover slips were incubated in a cocktail of primary antibodies (1: 100 in 4% BSA/TBST (w/v)) for one hour, followed by incubation in each secondary antibody (1: 100 in 4% BSA/TBST (w/v)) separately with PBS washes between each staining.

The binding sites were then extracted from these complexes.

The candidate binding sites are then identified on the basis of overlapping fragments (peak height) along the genome.

The binding sites are then predicted by building the energetically favorable ligand conformations from the predicted interaction hotspots.

Transcription factor (TF) binding sites are then predicted by detecting statistically significant TF binding sequences on the genome using a position weight matrix.

A pair of binding sites is then compared based on alignment of 90 lists of sorted distances obtained for each of the sites.

Putative binding sites are then considered to be functional if they are conserved in orthologous promoter sequences of multiple related species.