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Unspecific binding sites were subsequently blocked by incubating cells with 5% goat serum.
Functionally relevant binding sites were subsequently confirmed through chromatin immunoprecipitation.
Such binding sites were subsequently validated in detailed docking studies with known DAT ligands (56).
Nevertheless, several examples of such changes and the corresponding co-evolution of DNA binding sites were subsequently identified (e.g., [ 100]).
Similar(56)
Initially, models of information content of bacteriophage T7 RNA polymerase binding sites and other bacterial control systems were studied, and mRNA splice sites were subsequently developed.
All five sites were subsequently characterized, individually and combined.
To reduce the complexity associated with carrying forward multiple cavities per protein for BSC, the detected binding sites were ranked and subsequently filtered according to their potential to bind a small molecule ligand.
Specifically, a double-stranded DNA fragment containing three binding sites were synthesized, PCR-amplified and subsequently cloned into the MCS.
Subsequently, nonspecific binding sites were blocked by incubating the wells for 10 min with 1% goat serum (Sigma-Aldrich, Zwijndrecht, The Netherlands).
Subsequently, the plates were washed and free binding sites were saturated with phosphate-buffered saline (PBS)/0.05%Tween/3%3 % bovine serum albumin (BSA).
Potential binding sites were elucidated by docking.
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