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PACAP binding sites were studied using a radioreceptor assay with 125I-labelled PACAP in various tissues.
Rat brain guanosine binding sites were studied by (i) a pharmacological approach to confirm the hypothesis of the existence of specific G-coupled receptors for guanosine (1) and, for the first time, delineate a structure activity relationship for a series of guanosine derivatives; (ii) a molecular modelling approach to design a pseudo-receptor construction.
Additionally, the properties of binding sites were studied for mRNA sequences of both human and other animal species.
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Using these lines, the nature of integrin binding sites was studied in order to delineate the bioactivity of different collagen substrates.
The initial rate response of SULT1A1 to nucleotide binding at the high- and low-affinity sites was studied using 1-HP, a fluorescent acceptor.
Comparisons over the binding sites were specially studied to improve the discussion about the selectivity of potential new drugs.
However, polymorphisms in coding regions resulting in a non-conservative amino-acid substitution in conserved regions of the genome, or in potential transcription factor binding sites, are also studied because of their potential functionality.
A blast search for each primer pair verified that the binding sites were unique for each gene studied.
Of the transcription factors whose binding sites were predicted in this study, 37 had forty or more binding sites throughout the S. cerevisiae genome.
However, structural information for CMPKother, E2 31other and Survivinother sites are not publicly available, and the crystal structure of MurA is fosfomycin-covalent modified, so these 4 binding sites were excluded in our study.
The two undecanucleotides (11-mers) corresponding to the XerC and XerD binding sites were designated in this study as dif XerC and dif XerD, respectively, whereas the central hexanucleotide between the two Xer binding sites was named as dif cent (Figure 1A).
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binding sites were considered
binding sites were blocked
binding sites were determined
binding sites were defined
binding sites were generated
binding sites were retrieved
binding sites were used
binding sites were revealed
binding sites were selected
binding sites were identified
binding sites were extracted
binding sites were cloned
binding sites were designed
binding sites were shared
binding sites were shown
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