Sentence examples for binding sites were retained from inspiring English sources
This denotes that the Oct-1, CREB and possibly Sp1 are necessary for full cell specificity, because the Oct-1 but not the CREB and Sp1 binding sites were retained in the deletion.
Consistent with the species specificity of transcription factor binding sites, Rosen and colleagues recently compared PPARγ binding in 3T3-L1 andpocytes and in vitro differentiated primary human adipose stromal cells (hASC), and found that only 21.3% of the murine binding sites were retained in human adipocytes.
Here, we used ChIP-seq to generate genome-wide binding profiles of C/EBPα and PPARγ in human SGBS adipocytes, compared these to previously published profiles in mouse 3T3-L1 adipocytes [ 15, 16], and systematically studied what features affect whether a binding site is retained between species or not.
Hypomorphic mutation in the NBS1 gene in question, results in transcript truncated for the N-terminal portion thus lacking FHA and BRCT domains, whereas the C-terminal domain containing MRE11-binding and putative ATM-interaction motif sites are retained [ 23].
Age and study site were retained in the multivariate model.
While previous reports agree that retention of transcription factor binding sites is limited, systematic analysis of the biological features determining whether a site is retained or not has not been performed.
Residues defining the binding sites were selected for site-directed mutagenesis.
We then measured the fraction of mouse C/EBPα and PPARγ binding sites that was retained and lost in human.
The fraction of retained PPARγ binding sites is significantly higher in the vicinity of genes that are moderately or highly upregulated (clusters 3 and 4) compared to all other binding sites (Fischer Exact Test, P < 2.2*10^-16).
Importantly, we have shown that only two of the three binding sites are available in intact RAP, though they retain the very high affinities seen for isolated RAP domains to the respective (CR x species.
