Sentence examples for binding sites were mutated from inspiring English sources
When three or all four miR-137 binding sites were mutated simultaneously, the mutated vector alone was unable to express the luciferase gene (data not shown).
Full-length angiocidin bound polyubiquitin while three angiocidin recombinant proteins whose putative polyubiquitin binding sites were mutated either failed to bind polyubiquitin or had significantly diminished binding activity.
This Tcf21 promoter activity was significantly reduced when all three SRY binding sites were mutated.
When the 148/152B binding sites were mutated, the effects of miR-148 were eliminated.
A similar decrease in luciferase activity was observed when both AP-1 and c-Rel binding sites were mutated.
When the three putative binding sites were mutated simultaneously, the transactivation of TSP1 induced by E2F1 was abolished completely.
This repression does not decrease if the distal of the two binding sites is mutated (as the proximal site can still block transcription), but mutation of the proximal site results in a significant de-repression and loss of both sites completely abolishes transcriptional control by CysR.
Three 5′-flanking regions of the rat Vegf gene; 1) the construct including both C/EBPβ binding site and HIF1α binding sites (−1171 bp to +115 bp), 2) the construct lacking the C/EBPβ binding site (−976 bp to +115 bp) and 3) the construct that four HIF1α binding sites are mutated (−1171 bp to +115 bp), were amplified using the primer sets in Table 1.
As shown in Figure 7B and 7C, GFP was dramatically reduced when both the Pax2 and Sox2 binding sites are mutated, but not in the Pax2 or Sox2 single mutants (data not shown), indicating that both proteins are directly bound to the pou3f4 HCNR 81675 DNA region and required for its enhancer activity.
Moreover, the inhibition was partially rescued when one of the binding sites was mutated or almost fully relieved with all sites mutated.
It also tests whether this decrease is abrogated when the seed regions of the putative miR-34a binding sites are mutated, as seen in Figure 4D.
