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Transcription factor binding sites were mapped within hotspots using the P-Match program available from TRANSFAC [52].
The nucleotide modifications (by DMS), the pauses corresponding to nuclease (TI and VI) cleavage sites and the toe prints corresponding to PTB binding sites were mapped to MFOLD predicted structure for clarity (Fig. 3).
Using synthetic α-actinin-4 peptides, HAMLET binding sites were mapped to the N-terminal actin-binding domain and the proposed β integrin-binding central rod domain between spectrin repeats 1 and 2, suggesting that HAMLET might impair these essential interactions, [5], [6], [10].
Ribosome binding sites were mapped when possible according to the sequence described in reference [ 41].
Sox2 stable binding sites were mapped by low-excitation 2D single molecule imaging condition (Video 7 ).
TRANSFAC's 522 mammalian minimum false positive matrix profiles of binding sites were mapped to the promoter regions (see Methods).
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The TF binding sites are mapped onto the mm8 (February 2006) mouse assembly.
Interestingly, binding sites are mapped only in the intergenic regions (between rpmH– dnaA, dnaA– dnaN, yqeG– sda, ywlB– ywlC, jag– thdF, yycS– yydA, ywcI– vpr, and gcp– ydiF).
Genome wide analysis of ERα binding patterns has revealed that the largest fraction (38%) of ERα binding sites is mapped to intragenic regions including introns and several of these interactions have previously been described as unproductive [ 48- 50].
To use the entropy-based discretization, each track of data was sorted, and a list of known or predicted binding sites was mapped to the probe coordinate and used as the class label c of each data point.
When transcription factor binding sites are mapped on gene promoter regions, the anchoring transcriptional start site forms a separating point as shown in Figure 2. Upstream of the transcriptional start site, occurrence frequency of binding sites goes up, whereas the frequency is reduced downstream of the start site.
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