For amplification, two primer binding sites are included in the PLPs.
Each data set contains the relative binding affinities for nucleotide sequences with 52bp in length, in which shorter binding sites are included.
In the FoxA1 data, the qPCR validations supported further the good performance of MACSC when all the detections were used in the evaluation, while only one of the top 1000 binding sites was included in the set of validated regions.
The x-ray crystal structure contains several water molecules located either within or close to the binding site, hence we decided to carry out two separate docking runs; one in which water molecules within 5Å of the APO866 binding site were included in the calculations, and another without water molecules.
In addition, as the GSH binding site was included in the dimer interface of apo GrxS14 (Figs. 2B,2D and S8C), the binding of GSH is incompatible with the dimerization of GrxS14, which explains why GSH can prevent the dimerization of GrxS14 (Fig. S2B).
Open and closed conformations of the O helix were incorporated, and flipping of Tyr639 in and out of the NTP binding site was included.
For AANAT, only the region within the canonical 14-3-3 14-3-3 14-3-3is included in our analysis with the globindingegion being deleted.
A synthetic gene containing five copies of a perfect bpa-miR-5364 bpa-miR-5364 bpa-miR-5364 as a positive control in these experiments and gave a reduction in signal of 42% in the presence of binding-5364 versiteplasmid wash the mincludedert in the reverse orientasion (P = 0.0142, Mann–Whitney test) in the repositivetive experiment shown in Figure 5.
