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Exact(9)
All the designed mutant nucleotide sequences were re-analysed in Match 1.0 Public to check for loss of the specific transcription factor binding site and that no new binding sites were generated.
A binding p-value was then determined for each genomic position by Wilcoxon rank sum test and binding sites were generated from those more significant than specified thresholds with a maximum gap of 500 and minimum run of 350.
Two different types of sets of example binding sites were generated for each cutoff score from 2 to 7. They correspond to sequences drawn from a step-function distribution or a Boltzmann distribution, in each case using a maximum energy cutoff for selected sites.
Mutations in the miR-200c binding sites were generated by PCR directed mutagenesis.
For this purpose, either single mutations or the possible combinations of the potential binding sites were generated.
The potential ligand binding sites were generated using a probe radius of 5.0 Å and the binding site having highest z-score was considered for further investigation.
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A dataset of ligand-dependent binding sites was generated using the SiteHopper create tool [13] where default parameters create a binding site patch within 4 Å of a specified bound ligand.
A genome-wide map of predicted STAT1 binding sites was generated as follows.
After formation of the dityrosine bond a complete set of well-formed zinc binding sites is generated.
In order to decrease endogenous miR-181a-5p miR-181a-5p miR-181a-5puct with four repeats containing miR-181 binding sites was generated.
The plasmid pCAG-125b-6 pCAG-125b-6 pCAG-125b-6 copies of binding sites was generated using the isocaudamer of SpeI/ NheI.
More suggestions(21)
binding site were generated
binding sites were aggregated
binding sites were investigated
binding sites were integrated
binding sites were created
binding sites were assigned
binding sites were found
binding profiles were generated
binding sites were sought
binding conformations were generated
binding images were generated
binding sites were included
binding sites were compared
binding sites were refined
binding sites were identified
binding sites were isolated
binding sites were examined
binding sites were implicated
binding sites were blocked
binding sites were required
binding levels were generated
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