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However, structural information for CMPKother, E2 31other and Survivinother sites are not publicly available, and the crystal structure of MurA is fosfomycin-covalent modified, so these 4 binding sites were excluded in our study.
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To confirm this, rate limiting qPCR was performed using DNA immunoprecipitated from nitrogen limiting and excess conditions, and no enrichment was observed (data not shown), therefore this binding site was excluded from the GlnR regulon.
Insertions at previous jejunostomy tube sites were excluded.
These sites were excluded.
Blogs and advertisement-only sites were excluded.
GpCpG sites were excluded from analysis.
Regions 30 bases up- and downstream from the SNP site were excluded from being selected as PCR primer binding sites.
Nine OR genes (human genes: OR9G3P, OR11J2P, OR2AE1, OR56B4; mouse genes: MOR188-2, MOR126-2, MOR204-25PMOR176-3, MOR204-25P) were found to contain a gap within the putative binding site, and were excluded from subsequent analyses.
Residues defining the binding sites were selected for site-directed mutagenesis.
The possibility that Wg-stabilized Mad may bind to Armadillo/β-catenin, Pangolin/lef1, Legless/bcl9 and Pygopus independently of nearby Mad binding sites cannot be excluded at present.
Moreover, autoregulatory binding sites are rather predicted downstream of TSSs than in the further upstream bins (excluding the first upstream bin).
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